Figure 3. Immunodetection of wild type and mutant CX50 in transfected HeLa cells. A, B: HeLa cells transfected with CX50 or CX50I247M were fixed 48 h after transfection and subjected to immunofluorescence using anti-CX50 antibodies. The distribution of CX50 immunoreactivity appeared similar in both groups of cells; cells expressing either CX50 or CX50I247M showed a significant number of gap junctional plaques (arrows). The scale bar represents 13 μm in A and 17 μm in B.