Figure 4. Decursin induced no cytotoxicity in retinal endothelial cells and no retinal toxicity. A: Human retinal microvascular endothelial cells were treated with 1–100 µM of decursin then incubated for 48 h. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Each value represents means±SEM from three independent experiments. The asterisk indicates a p<0.05. B: We injected intravitreously 50 µM decursin into mouse eyes, then enucleated the globes three days after treatment. Hematoxylin & eosin staining and immunohistochemistry for glial fibrillary acidic protein were performed. Figures were selected as representative data from three independent experiments with similar results. Scale bars equal 50 µm. Abbreviations: ganglion cell layer (GCL), hematoxylin and eosin (H&E), inner nuclear layer (INL), outer nuclear layer (ONL).