Figure 2. Decursin inhibits VEGF-induced migration and tube formation of HRMECs. A: Human retinal microvascular endothelial cells (HRMECs) were plated onto gelatin-coated culture dishes and wounded with a razor blade. Wound was made by scraping across with the razor blade. The wounded monolayers were treated with incubation in 10 µM decursin or 20 ng/ml vascular endothelial growth factor (VEGF) for 12 h. Figures were selected as representative data from three independent experiments. Migration was quantified by counting the number of cells that moved beyond the reference line. The basal migration of HRMECs that were left without decursin and VEGF were normalized to 100%. Each value represents the mean (±SD) of three independent experiments. The asterisk indicates a p<0.05. B: HRMECs were inoculated on the surface of the basement membrane matrix and treated with 10 µM decursin or 20 ng/ml VEGF for 18 h. Figures were selected as representative data from three independent experiments. The basal tube formation of HRMECs without decursin and VEGF was normalized to 100%. Each value represents the mean (±SD) of three independent experiments. The asterisk indicates a p<0.05.