Figure 1. Decursin inhibits VEGF-induced migration and tube formation of HRMECs. A: Human retinal microvascular endothelial cells (HRMECs) were treated with 20 ng/ml vascular endothelial growth factor (VEGF) or 1–50 µM decursin for 24 h. A cell-proliferation assay with [³H]-thymidine was performed. Each value represents the mean (±SD) of three independent experiments. The asterisk indicates a p<0.05. B: HRMECs were treated with 20 ng/ml VEGF or 10 µM decursin for 5 min. Western blot analysis using phospho- (p-)VEGFR-2 and vascular endothelial growth factor receptor-2 (VEGFR-2) antibodies was performed, with β-actin serving as the loading control. Figures were selected as representative data from three independent experiments. Quantitative analysis was performed by measuring the intensity relative to the control. Each value represents means±SEM from three independent experiments. The asterisk indicates a p<0.05. The size of scale bars in figure A, B were 100 µm.