Figure 9. Different CDH23 antisera recognize different protein isoforms. Western blot analyses of protein extracts from P10 and P90 mouse inner ears and retinas were incubated with antisera TF7 and 1D1 generated against mouse and human CDH23 cytoplasmic domain respectively. The highest molecular weight band detected by antisera TF7 (arrow) possibly represents the longest CDH23 isoform (CDH23_V1) expressed in mouse inner ear. The same band was not detected using antisera 1D1 in the inner ear or retina. Antisera 1D1 recognized bands just above (asterisk) and below (two asterisks) the 210 kDa size marker. The band under 210 kDa was also detected by antibody TF7. An additional band (arrowhead) was detected by antisera 1D1, which seems to be detected by antisera TF7 or a band very close in size to it in the inner ear. Antisera TF7 detected a band of roughly 100 kDa that may correspond to CDH23_V2. Additional bands of lesser molecular weights were detected by both antisera, possibly representing smaller still uncharacterized CDH23 protein isoforms.