Figure 8. Primate and mouse CDH23 protein in inner ear and retina. A-C: western blot analyses of proteins separated on 3%–8% tris-acetate gels using the cytoplasmic domain antibody, TF7. A: Western analysis, using protein extracts from P0, and P90 wild-type and mutant mouse inner ear and retina. An approximately 350 kDa band corresponding to the largest CDH23 protein isoform was only detected in P0 mouse inner ear (arrow head). Faster migrating protein bands were present in wild-type and Cdh23^v-6J and may represent lower molecular weight CDH23 protein isoforms (such as CDH23_V3). Tissue specific variation of these isoforms is better visualized in the western blot shown in panel B. B: Western analysis, using protein extracts from P4, and P60 wild-type mouse inner ear and retina. The high molecular weight band at roughly 350 kDa (arrowhead) was detected in the P4 inner ear protein sample. Traces of this band were also detected in the P60 inner ear protein sample. The faster migrating bands detected show variability in their appearance between young and adult tissue as well as variability between the inner ear and retina. C: Western analysis, using protein extracts from P0 mouse retina, human retina, and monkey retina. The high molecular weight band (arrow head A-C) detected in P0 wild-type mouse inner ear corresponds in size to the largest band detected in human and monkey retinas (arrowhead). β-actin was used as a loading control; size standards are given in kDa.