Figure 6. CDH23 localizes with SNAP25 to the synaptic region of mouse auditory and vestibular hair cells. In A-I nuclei were stained with DAPI (blue). SNAP25 is shown in red, and CDH23 in green. A: The synaptic region of utricular hair cells stained with SNAP25. B: CDH23_V3 detected with antisera TF648. C: In the merge image (C) of panels A and B, CDH23_V3 shows partial colocalization with SNAP25 in the synaptic region of utricular hair cells. The insets in A-C show a twofold magnification of a hair cell (asterisk) synaptic area. In the merge image (F) of panels D and E, CDH23_3 and SNAP25 show partial colocalization in the synaptic region of inner (IHC) and outer (OHC) hair cells. G: SNAP25 staining of IHC and OHC synaptic fibers. H: CDH23 is detected with antiserum TF7 in the synaptic fibers of IHC and OHC. I: In the merge image (I) of panels G and H, CDH23 shows partial colocalization with SNAP25 in the region of the synaptic fibers of IHC and OHC. In G-I, the insets are a twofold magnification of the region (asterisk), where CDH23 localizes in the synaptic fibers. Scale bars equal 5 µm.