Table 1 of Petersen-Jones, Mol Vis 2009; 15:1835-1842.
Table 1. Details of primary antibodies used for IHC
Antibody |
Host |
Target cell |
Working dilution |
Source |
---|---|---|---|---|
Anti-GFP | Rabbit | Cells expressing GFP protein | 1:5,000 | Dr. Luc Berthiaume, University of Alberta, Alberta, Canada |
Human Cone Arrestin | Rabbit | Cone photoreceptors | 1:100,000 | Dr. Cheryl
Craft & Xuemei Zhu, Mary Allen Lab, Doheny Eye Institute, University of Southern California, Los Angeles, CA |
Protein Kinase C alpha | Mouse | Rod bipolar cells | 1:200 | BD Biosciences, PharMingen, Rockville, MD |
PSD95 | Mouse | Rod spherules and cone pedicles | 1:100 | Neuromab, University of California Davis, Davis, CA |
Calbindin | Mouse | Horizontal cells | 1:1,000 | Swant Immunochemicals, Bellinzona, Switzerland |
Calretinin | Rabbit | Horizontal cells | 1:1,000 | Swant Immunochemicals, Bellinzona, Switzerland. |
GFAP | Rabbit | Astrocytes and reactive Müller glia | 1:2,000 | DakoCytomation, Carpinteria, CA |