Figure 3. AAV2/5-GFP transduction includes a few horizontal cells and Müller glia. A: The panel shows native GFP fluorescence (scAAV2/5-GFP-injected retina), calbindin and calretinin immunohistochemistry and a merge of all three. The arrow indicates a horizontal cell that is expressing GFP. B: The image on the left shows endogenous GFP expression; some inner retinal cells are expressing GFP (ssAAV2/5-GFP-injected retina). The image on the right shows immunohistochemistry using an anti-GFP antibody. The arrows indicate Müller cells that are expressing GFP. Scale bar equals 50 µm.