Figure 5. RhoA activation is involved in hypoxia inhibition. Cells were treated under normoxia (control) or hypoxia (H) with or without 5 ng/ml TGFβ1 for 7 min. GTP bound RhoA was pulled down and assayed by western blot with anti-RhoA antibody. Total RhoA from whole cell lysates was detected as the relative control. The representative image of three experiments is shown here. Error bars represent standard error of the mean (n=3). The asterisk denotes that the indicated groups were significantly different from TGFβ1 (p<0.05).