Figure 4. Hypoxia inhibition of TGFβ1-induced α-SM actin is independent of MAPK activation. A: Whole cell lysates were collected 4 h after treatment and assayed by western blot for pERK, total ERK, pJNK, and phospho-p38 as indicated. Vimentin was used as a loading control. B: The bar graph shows the relative increase of pERK in each group over the control. Error bars represent standard error of the mean (n=3). The asterisk indicates that the indicated groups were significantly different from control (p<0.05).