Figure 3. Hypoxia does not reduce TGFβ1 induced interaction between pSmads and CBP in rabbit keratocytes. Nuclear extracts were collected 4 h after corresponding treatment and immunoprecipitated with anti-CBP antibody. Eluates were separated by SDS–PAGE and probed for pSmad3. Blots were also probed for CBP as an internal control. A: Image shows a representative western blot. B: Bar graph shows the relative change of pSmad3 over the control group. Error bars represent the standard error of the mean (n=3). The asterisk denotes that the indicated groups were significantly different from control (p<0.05).