Figure 2. Hypoxia treatment reduces TGFβ1 induced α-SM actin but not Smad3 phosphorylation. Primary isolated rabbit keratocytes were treated with hypoxia together with or without TGFβ1 for 4 h. Whole cell lysates were collected immediately after treatment and analyzed by western blot for α-SM actin (A), pSmad3 (B), and totalSmad2/3. Vimentin was used as a loading control. Bar graphs show fold increase of band intensity of each group compared to vimentin (A) and to total Smad2/3 (B). Error bars represent the standard error of the mean (n=3). The asterisk denotes p<0.05.