Figure 8. Western blot analysis of the
wild-type and mutant CLRN1-HA polypeptides. BHK-21 cells were
transfected with the indicated HA-tagged CLRN1 plasmids. Nontransfected
cells (0-BHK) were used as controls. Polypeptides were resolved on 12%
SDS–PAGE, and anti-HA antibodies were used to probe the blots. Samples
were untreated (-) or treated (+) with deglycosylating enzyme (PNGase
F). The molecular weights of the protein bands are indicated on the
left and right sides of the figure.