Figure 6. Cellular localization of the p.L54P, p.N48K and WT CLRN1-HA polypeptides. The transfected BHK-21 cells were immunostained with HA antibody (red) showing the localization of WT CLRN1-HA (E), the novel sequence alteration p.L54P mutated CLRN1-HA (B) and the known disease-causing p.N48K mutated CLRN1-HA (H). The same cells were immunostained with plasma membrane –specific antibody (green) in panels A, D, and G. Double-staining shows that WT CLRN1-HA (F) and the p.L54P mutated CLRN1-HA (C) colocalize (yellow) with the plasma membrane marker whereas the known mutation p.N48K (I) does not colocalize with the plasma membrane marker. Cells were viewed with a confocal immunofluorescence microscope. Scale bar represents 10 µm.