Figure 5. Cellular localization of the disease-causing mutant CLRN1-HA polypeptides. The transfected BHK-21 cells were double immunostained with HA antibody (red) in panels B, E, H, K, and N showing the localization of mutant CLRN1-HA. In panels A, D, G, J, and M the cells were stained with the ER marker (green). The right-most panels C, F, I, L, and O show the overlay of the mutant CLRN1- HA staining (red) and ER-specific staining (green). Yellow-orange staining indicates colocalization of these stainings. Cells were viewed with a confocal immunofluorescence microscope. Scale bar represents 10 µm.