Figure 4. Cellular localization of WT CLRN1-HA protein in transfected BHK-21 cells. In panels B and E the cells were immunostained with HA antibody (red). In panel A the cells were immunostained with a plasma membrane specific antibody (green) and in panel D with ER specific antibody (green). The right-most panels (C and F) show the overlay of both CLRN1-HA and the organelle-specific double staining. Yellow-orange staining indicates an overlap of the CLRN1-HA protein (red) and subcellular markers (green). Cells were viewed with a confocal immunofluorescence microscope, magnification 63×. Scale bar represents 10 μm.