Table 1 of Zenteno, Mol Vis 2009; 15:1794-1798. Table 1 of Zenteno, Mol Vis 2009; 15:1794-1798.
Table 1. PCR primers for MFRP gene amplification
Exon |
Oligonucleotide sequence (5′-3′) |
Annealing temperature (°C) |
PCR product size (bp) |
|---|---|---|---|
| 1–4 F | GGTCTTGGGCTGTCACAGG | 60.3 | 843 |
| 1–4 R | CCACCCCGTCATCTTGGGC | ||
| 2 R | CCTTCTGTTGGGTATTCCTC | (for sequencing only) | |
| 5 F | CTTGAGAATAAAGGACCTCA | 59.5 | 327 |
| 5 R | CTTTAGATAGTGGTTCAGGA | ||
| 6 F | ATAGGACTGCAAGGCCCAGG | 58.7 | 287 |
| 6 R | CCTTTGACAGGACTGGGAGT | ||
| 7–8 F | ACTCCCAGTCCTGTCAAAGG | 59.2 | 582 |
| 7–8 R | TTCCCATTACACTAACTTGG | ||
| 9 F | TTAAGAACCACCAATGATG | 54.6 | 370 |
| 9 R | TGGAGAATGGAATGTGCTGG | ||
| 10 F | AGCTCAGAGCCAGGCCTGT | 58.2 | 264 |
| 10 R | CCTGGAGGTGCCTCTACT | ||
| 11 F | GGACAGACAAGGGCTCTGGA | 59.1 | 304 |
| 11R | ACTGTGCAGTACGGCAGTAGG | ||
| 12 F | ATTCGGTGACTTGCCACAGG | 59.1 | 581 |
| 12 R | TTGTTCCCCTGCGTGCCAGC |