Table 1 of Zenteno, Mol Vis 2009; 15:1794-1798.
Exon |
Oligonucleotide sequence (5′-3′) |
Annealing temperature (°C) |
PCR product size (bp) |
---|---|---|---|
1–4 F | GGTCTTGGGCTGTCACAGG | 60.3 | 843 |
1–4 R | CCACCCCGTCATCTTGGGC | ||
2 R | CCTTCTGTTGGGTATTCCTC | (for sequencing only) | |
5 F | CTTGAGAATAAAGGACCTCA | 59.5 | 327 |
5 R | CTTTAGATAGTGGTTCAGGA | ||
6 F | ATAGGACTGCAAGGCCCAGG | 58.7 | 287 |
6 R | CCTTTGACAGGACTGGGAGT | ||
7–8 F | ACTCCCAGTCCTGTCAAAGG | 59.2 | 582 |
7–8 R | TTCCCATTACACTAACTTGG | ||
9 F | TTAAGAACCACCAATGATG | 54.6 | 370 |
9 R | TGGAGAATGGAATGTGCTGG | ||
10 F | AGCTCAGAGCCAGGCCTGT | 58.2 | 264 |
10 R | CCTGGAGGTGCCTCTACT | ||
11 F | GGACAGACAAGGGCTCTGGA | 59.1 | 304 |
11R | ACTGTGCAGTACGGCAGTAGG | ||
12 F | ATTCGGTGACTTGCCACAGG | 59.1 | 581 |
12 R | TTGTTCCCCTGCGTGCCAGC |