Figure 6 of Govardovskii, Mol Vis 2009; 15:1717-1729.

Figure 6. Equilibration of rhodopsin and its bleaching products can be incomplete. A: A shows absorbance difference between unbleached and bleached side after half-field bleach. B: Spectra at 11 min postbleach showed that an extra rhodopsin (peak at roughly 515 nm) remained in the unbleached ROS half while photoproducts (retinal and Meta II, peak at 380 nm) stayed predominantly in the bleached half. Nonequilibrating rhodopsin fraction comprised 41% of original concentration difference. Measurements are made on salamander ROS in Ringer pH 6.3.