Figure 5. Tracing rhodopsin diffusion at
physiologic pH in the presence of hydroxylamine. Oblique arrow in A
points to initial absorbance increase at the bleached side due to the
formation of extra Meta I-like product in hydroxylamine-containing
solution. Multiphase absorbance difference changes seen up to 30 s
postbleach in B were due to formation of metaproducts and their
subsequent conversion to retinaloxime. ROS diameter is 11.6 μ, the
apparent diffusion constant Da=1.3×10−9 cm2s−1.