Figure 4. Predominant expression of Chop2-GFP in AII amacrine cells. A: In the retinal whole-mount, GFP-positive AII amacrine cells were found throughout the retina. B, C: At high magnification in the retinal whole-mount, GFP expression was detected in the somas of AII cells in the INL (B) and in the arboreal dendrites in the IPL (C). D: In the retinal vertical section, many AII amacrine cells were GFP-positive. E-G: In the same retina shown in D, GFP-positive cells (E) were immunolabeled for Disabled-1 (F). The merge image is shown in G. Double-labeled AII amacrine cells are marked by arrows. For A-C, GFP fluorescence was enhanced with antibodies against GFP. Abbreviations: outer nuclear layer (ONL); outer plexiform layer (OPL); inner nuclear layer (INL); inner plexiform layer (IPL); ganglion cell layer (GCL). Scale bars equal 500 μm in A, and 25 μm in B-D.