Figure 2. Long-term expression of Chop2-GFP in different retinal cell types. The images were taken from the retina of a 20-month-old mouse that was injected with the virus at the age of 3 months. In the left eye (A, B), numerous ganglion cells can be seen in the whole-mount. In addition, AII amacrine cells (arrows) and horizontal cells (asterisks) were visible in a vertical section of the same retina (C). In the right eye (D, E), many horizontal cells that express the Chop2-GFP construct were found in the whole-mount (D). In the vertical section, photoreceptors and horizontal cells (asterisks) showed GFP fluorescence (E). In C and E, GFP fluorescence was enhanced with antibodies against GFP. Abbreviations outer nuclear layer (ONL); inner nuclear layer (INL); inner plexiform layer (IPL); ganglion cell layer (GCL). Scale bars equal 50 μm in A, B, and D, and 25 μm in C and E.