Figure 3. Hydrostatic pressure-induced phosphorylation of CREB is inhibited by H-89 in AA astrocytes. A: Shown is a representative western blot of p-CREB (Ser-133) phosphorylation and total CREB (t-CREB) in AA and CA astrocytes treated with 3h of elevated HP, IBMX, and HP + H89 (PKA inhibitor). Treatments do not alter total CREB in either cell type, but p-CREB phosphorylation is inhibited by H89 only in AA astrocytes. B: Bar graph of p-CREB/t-CREB (normalized to control=1.0) under various conditions for AA astrocytes (n=4) and panel C. CA astrocytes (n=3). Error bars indicate SEM. The change in p-CREB is significant (p<0.05, asterisk, n=3) in astrocytes treated with either pressure or IBMX.