Table 2 of Lin, Mol Vis 2009; 15:1655-1663.


Table 2. Conditions of PCR and HRM. The primer pairs, condition, product size, and restriction enzyme of TGF-β2 (rs991967), bFGF (rs308395) in the PCR-RFLP and TGF-β2 (rs7550232) and bFGF (rs41348645) in the reaction of HRM reaction.


Sequence
Polymorphism
(rs number)



Primer
PCR
Product
size
(bp)
PCR
conditions
(annealing
temperature)

Restriction
enzyme
site

Alleles
DNA
Fragment
size
(bp)
TGF-β2rs991967 F: 5′-TGACCGAGAAAGTCTGCATT-3′ 239 55 °C HpyCH4III A 239
  R: 5′-AAGGTCTGAAGTTTGACCAGTACA-3′       C 100+139
bFGFrs308395 F: 5′-GCATGGCCTTTTGAAACCTA-3′ 241 55 °C BsrI C 241
  R: 5′-CAGCGTCTCACACACTGAGG-3′       G 163+78
FMODrs7543148 F: 5′-GCTGG CTTGC TCTGT TCTCT-3′ 1033 55 °C BsmI G 1033
  R: 5′-GCCAA GGTCT CACCA TTGAT-3′       A 751+282

Sequence
Polymorphism
(rs number)



Primer


DNA
concentration


Mg
concentration
PCR
conditions
(annealing
temperature)



Alleles
TGF-β2rs7550232 F: 5′-AACGGGAGACTTGATTGTCCT-3′ 7.8 ng 2 mM touchdown 60/53 °C A
  R: 5′-CGAACCGTTGAGGGAGTGT-3′       C
bFGFrs41348645 F: 5′-ACCATAGACTGTCTTACCCA-3′ 7.8 ng 2 mM touchdown 60/53 °C A
  R: 5′-CAATTGTAAGGGAAGTCAGC-3       G