Figure 4. DNA sequence analysis of OPTN exon 10. A: The forward sequence of OPTN exon 10 in the glaucoma family. An ‘AAA’ to ‘GAA’ transition at amino acid Lys322Glu in exon 10 is observed. The underlined base is the location of mutation. B: The forward sequence of OPTN exon 10 in normal controls. C: The reverse sequence of OPTN exon 10 in the glaucoma family. D: The reverse sequence of OPTN exon 10 in normal controls.