Figure 3. Diff-Quik staining photos and ex vivo confocal microscopy scans of the brush cytology specimens from patients with atopic keratoconjunctivitis. A and B: Corresponding brush cytology photos and confocal microscopy scans from a representative 12-year-old female patient. Confocal microscopy could effectively discern the nuclear details such as segmentation in polymorphs (yellow arrow heads), epithelial cell clumps (yellow arrows), nuclei in conjunctival epithelial cells (which appeared as round hyperreflective oval bodies) and the mucin blots appeared as hyperreflective bodies with similar/exact shapes resembling the Diff-Quik stained specimens (blue arrows). Corresponding brush cytology photos and confocal microscopy scans from a representative 17 year old male patient are also shown in C and D. Confocal microscopy could effectively discern the nuclear details such as segmentation in polymorphs (yellow arrow heads), and the double nuclei in eosinophils (orange arrow heads) with similar/exact shapes resembling the Diff-Quik stained specimens.