Table 2 of Wang, Mol Vis 2009; 15:181-186.

Table 2. Primers used for evaluating the variations by heteroduplex-single strand conformational polymorphism analysis.




Variations


Primer sequence (5'-3')		 Size of
PCR
product
(bp)
Annealing
temp
(°C)
c.192C>G F-TGCCCTCAGCCCTCAAGTATC 340 66
R-CAGCCCAAGCAGCAGGAGGAG

c.55-14_55-13insGTAT F-CTTTTCCCTCGGGTAGTAGA 344 62
R-GACGCTGTAGCTGGCATCCT

c.664C>A F-TCTCTGGCTGACCCTGCTCTT 152 60
c.669G>A R-AAACCAAATCCTTCCACACTGCT

c.770G>A F-TTGGGGGTTGAGAAAATAGG 283 60
R-TGGGTGGAGGGGAAGAAAGTG

In the "Primer sequence" column, "F" indicates the forward sequence and "R" indicates the reverse sequence.

Wang, Mol Vis 2009; 15:181-186. http://www.molvis.org/molvis/v15/a17

©2009 Molecular Vision http://www.molvis.org/molvis/

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