Table 1 of Wang, Mol Vis 2009; 15:181-186. Table 1 of Wang, Mol Vis 2009; 15:181-186.
Table 1. Primers used for polymerase chain reaction amplification and sequencing of MFRP.
Exon |
Primer sequence (5'-3') |
Size of PCR product (bp) |
Annealing temperature (°C) |
Note |
|---|---|---|---|---|
| 1,2 | F-CTTTTCCCTCGGGTAGTAGA | 466 | 60 | GC buffer |
| R-GTTGGCAGGTGGGGTTTTGAA | ||||
| 3,4 | F-AGGACAGCATGAGGAATACC | 504 | 62 | |
| R-AACCCCACCCCGTCATCTTG | ||||
| 5 | F-GAGAAGGGCCCAAGATGACG | 405 | 63 | GC buffer |
| R-TCCCTGCCACTCCCTGATTC | ||||
| 6,7 | F-TTGGGGGTTGAGAAAATAGG | 601 | 58 | |
| R-CTGGGCCAAAGAATGACTGA | ||||
| 8 | F-ATCACCGCCAGCCCTATTG | 281 | 62 | |
| R-CATCCCCCGTCTGCTTGAT | ||||
| 9 | F-GGTGCCCGGGATGAGACAG | 267 | 62 | GC buffer |
| R-CCGGGGGTGGCAGACAGT | ||||
| 10,11 | F-GCAGTGCCCCCTCAGTCAGC | 511 | 64 | |
| R-GTGGGCACCCAGCCTGCTC | ||||
| 12,13 | F-CAGGCCACAGAGCCAGTGAG | 548 | 63 | |
| R-AGCCCTGACCGGCAAAAGAG |