Figure 2. rAAV-mediated human IL-1Ra transgene expression in rabbit eyes. The vitreal cavity of rabbit eyes was injected with rAAV-IL-1Ra (4×10⁷ infectious units), and the contralateral eye was injected with the same amount of rAAV-LacZ or PBS as a control. Ten days later, the rabbit eyes were removed and processed for immunohistochemistry. In rAAV-IL-1Ra-injected eyes, transgene expression was found in various ocular tissue cell types, including retinal ganglion cells (A, arrowhead), retinal pigment epithelial cells (A, arrow), and ciliary epithelial cells (C, arrowhead). No human IL-1Ra transgene expression was detected in the control eyes (B and D). The scale bar in D is equal to 25 μm.