Table 1 of McDonald, Mol Vis 2009; 15:1509-1520. Table 1 of McDonald, Mol Vis 2009; 15:1509-1520.
Table 1. Primer pair sequences for real-time RT–PCR.
| Gene name (abbreviation, accession number) | Primer Sequence (5′-3′) |
|---|---|
| 18S | F: CTTAGAGGGACAAGTCGCG |
| R: GGACATCTAAGGGCATCACA | |
| 28S | F: TTGAAAATCCGGGGGAGAG |
| R: ACATTGTTCCAACATGCCAG | |
| Matrix Metalloproteinase 1 (mmp1 -AF134714) | F: ACTTGTACCGGGTGGCAGCG |
| R: TGGGATTTTGGGAAGGTCCG | |
| Matrix Metalloproteinase 2 (mmp2 - NM_174745) | F: ACTTCTTCCCCCGAAAGCCC |
| R: GGCACGAGCGAAGGCATCAT | |
| Matrix Metalloproteinase 14 (mmp14 -NM_174390) | F: TTCCATGGTGACAGCACGCC |
| R: ATGGCCCAGCTCGTGCACAG | |
| Tissue Inhibitor Metalloproteinase 1 (timp1 - NM_174471) | sense 5′-TCCACAGGTCCCAGAACCGC-3′ |
| antisense 5′-CATGCTGTTCCAGGGAGCCA-3′ | |
| Tissue Inhibitor Metalloproteinase 2 (timp2 - NM_174472) | sense 5′-ACTCGATGCCCCATGATCCC-3′ |
| antisense 5′-GCAGGAGCCGTCGCTTCTCT-3′ | |
| Collagen IV (coll IV M63139) | sense 5′-GGTTGATGGGAGAGCCTGGC-3′ |
| antisense 5′-ATCACCTCTGGCCCCTGGCT-3′ |