Figure 5 of McDonald, Mol Vis 2009; 15:1509-1520.


Figure 5. AGE-FN induce O2- production in RMEC. The lucigenin-enhanced chemiluminescence assay was used to determine O2- in RMECs. A typical trace is shown for RMECs growing on native FN or AGE-FN (A). Quantification revealed a significant increase in O2- when cells were growing on AGE-FN (B). Cells stimulated with NADPH showed a significantly enhanced O2- production (C) with the RMECs growing on AGE-FN, producing significantly more radicals compared to controls (D; n=3; **p<0.01).