Figure 6. Measurements of the percentage of cell membrane area specialized as gap junctions from the outer cortex to the inner cortex. Quantitative measurements of the percentage of cell membrane area specialized as gap junctions from the outer cortex (0-400 μm from surface) to inner cortex (400-800 μm from surface). The area of gap junctions is significantly reduced by approximately 5 times from the outer cortex (~33%) to inner cortex (~6%). A total of 539 junctions were counted in three replicas in the outer cortex, and 141 gap junctions in three replicas in the inner cortex.