Figure 3. Structural analysis of the mutant protein. The predicted three-dimensional protein structures for CRYBB2^WT (A) and CRYBB2^V187M (B), without side chains. The electrostatic potential is displayed in red (negative potential) and blue (positive potential) clouds. The mutant protein exhibits an unusually large negative electrostatic potential (green arrow). The amino acid V187 in the wild type is marked in red and in the mutant form, M187 is marked in green. Kyte-Doolittle hydropathy plot of CRYBB2^WT (C) and CRYBB2^V187M (D). X-axis represents position of amino acids. Y-axis represents hydropathy value in a default window size of 7.The region of interest is marked by white boxes. The increase in hydrophilicity in the mutant form is evident.