Table 2 of Wyatt, Mol Vis 2009; 15:1445-1448.

Table 2. List of oligonucleotides used in the MLGA protocol.

Oligonucleotide Sequence (5′→3′)
SOT2OT CTCTTAGCTTGGTTTCCTCCAGTCCACGATAACGGTAGAAAGCTTTGCTAACGGTCGAGTTTTGAAAACAGACGATAGAAGTCT
OTX2 CCAATTCACTCCCCCCTCTAGCACACGATAACGGTAGAAAGCTTTGCTAACGGTCGAGCAGAATGGAGGTCAAAACAAAGTGA
NPM1 GTCCCGCCTCCGCGCGACGATAACGGTAGAAAGCTTTGCTAACGGTCGAGCTCATGTCCATGTCCATCGAATCTT
Vector CTCGACCGTTAGCAAAGCTTTCTACCGTTATCGT
Forward primer AGCTTTGCTAACGGTCGAG
Reverse primer AGCTTTCTACCGTTATCGT

Table showing oligonucleotide sequences for additional selector probes (SOX2OT, OTX2 and NPM1) not described in the original MLGA report by Isakson et al. [12], the vector used during circularisation and the universal primers used for multiplex amplification.

Wyatt, Mol Vis 2009; 15:1445-1448. http://www.molvis.org/molvis/v15/a153

©2009 Molecular Vision http://www.molvis.org/molvis/

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