Figure 2. SDS-gel electrophoresis of the purified wild-type αB-crystallin and its mutants. αB-Crystallin and its mutant proteins were purified and the purity was checked by SDS-PAGE. Lane M, molecular mass markers. The masses of reference marker proteins are 94 kDa, 66 kDa, 45 kDa, 30 kDa, 20 kDa, and 14 kDa (from top to bottom of the gel), respectively. A: Lane 1, WT; Lane 2, Δ2; Lane 3, Δ5; Lane 4, Δ7; Lane 5, Δ10; and Lane 6, Δ12. B: Lane 1, Δ1; Lane 2, Δ1/K174A; Lane 3, Δ1/K174S; Lane 4, Δ1/K174E; and Lane 5, Δ11. C: Lane 1, K174A; Lane 2, K175A; Lane 3, K174/175A; and Lane 4, K174/175E. Coomassie brilliant blue-stained SDS gel shows essentially pure proteins for these crystallins (1-3 μg protein per lane).