Figure 6. Upregulation of NOS2 in retinal CD11b⁺ cells after ischemia or reperfusion. One eye from each mouse was subjected to ischemia-reperfusion (I-R). Nonischemic eyes were used as controls. Eyes were obtained seven days after I-R. Retinal cell suspensions were stained with isotype control or an anti-CD11b mAb followed by permeabilization and staining with anti-NOS2 antibody. Expression of NOS2 (cMFI) was calculated on gated CD11b⁺ cells. Data are shown as the mean±SEM from three independent experiments.