Figure 5. Upregulation of ICAM-1 on CD105⁺ and vimentin⁺ cells after ischemia or reperfusion. One eye from each mouse was subjected to ischemia- reperfusion (I-R). Nonischemic eyes were used as controls. Eyes were obtained 1 day after I-R. Retinal cell suspensions were stained with isotype control antibodies, anti-CD105, or anti-vimentin antibodies plus an anti-ICAM-1 antibody. Expression of ICAM-1 (cMFI) was calculated on gated CD105⁺ and vimentin⁺ cells. Data are shown as the mean±SEM from three independent experiments.