Molecular Vision: Kaur, Mol Vis 2009; 15:1366-1373. Table 1

Table 1. FOXC1 PCR amplifications and primer details.

Amplicon	Reaction conditions	Annealing temperature	Forward primer sequence	Reverse primer sequence	Fragment length (bp)	Genomic position	Region
FOXC1i	FailSafe – G	56.5	CGGTTCTCACCTCCCATTG	TTGACGAAGCACTCGTTGAG	1045	chr6: 1555048–1556092	Exonic
FOXC1ii	FailSafe – K	60	AGTTCATCATGGACCGCTTC	ACGTACCGTTCTCGGTCTTG	381	chr6: 1555996–1556376	Exonic
FOXC1iii	FailSafe – J	61	GCATCCAGGACATCAAGACC	CAAGTGGCCCAGGTCTCC	791	chr6: 1556344–1557134	Exonic
FOXC1iv	Qiagen	60	CTCACCTCGTGGTACCTGAAC	AGAGTTTTCTTCGTGCTGGTG	441	chr6: 1557087–1557527	Exonic/ 3′-UTR
FOXC1v	Qiagen	60	TCCCTCCAAAAATTCAGCTC	ACGTCAGGTTTTGGGAACAC	434	chr6: 1557482–1557915	3′-UTR
FOXC1vi	Qiagen	60	TGGATGTCGTGGACCAAAC	CTAGCCTCAAAGCAAGCTGAC	414	chr6: 1557869–1558282	3′-UTR
FOXC1vii	Qiagen	59	TTTATTTTCCTGCAGCATCTTC	GATTAAATATCCCTTTCCAACC	462	chr6: 1558222–1558680	3′UTR
FOXC1viii	Qiagen	59	TCCCCCATTTACAATCCTTC	AATCACAGGCCACGTAGAGC	557	chr6: 1558597–1559153	3′UTR