Table 1 of Chen, Mol Vis 2009; 15:1359-1365. Table 1 of Chen, Mol Vis 2009; 15:1359-1365.
Table 1. Primers used for mutation screening.
| Gene symbol and amplified fragments |
Primer sequences |
PCR product size (bp) |
Annealing temperature (°C) |
|---|---|---|---|
| CRYAA | |||
| Exon 1 | F:5′-CTCCAGGTCCCCGTGGTA-3′ | 251 | 65 |
| R:5′-AGGAGAGGCCAGCACCAC-3′ | |||
| Exon 2 | F:5′-CTGTCTCTGCCAACCCCAG-3′ | 220 | 65 |
| R:5′-CTGTCCCACCTCTCAGTGCC-3′ | |||
| Exon 3 | F:5′-GGCAGCTTCTCTGGCATG-3′ | 309 | 65 |
| R:5′-GAGCCAGCCGAGGCAATG-3′ | |||
| CRYAB | |||
| Exon 1 | F:5′-TGCATATATAAGGGGCTGGCTGTA-3′ | 363 | 65 |
| R:3′-CAGGGTAGGAAAGGAAAATGGATG-3′ | |||
| Exon 2 | F:5′-AGGATGAATTACCCGGACAGAAAG-3′ | 220 | 60 |
| R:5′-ACCCCTGATCCCGACTGTTAT-3′ | |||
| Exon 3 | F:5′-TGAGTTCTGGGCAGGTGATAATAGTT-3′ | 273 | 60 |
| R:5′-AGCTTGATAATTTGGGCCTGCC-3′ | |||
| CRYBA1/A3 | F:5′-CAATCCTCCCTCCACCTC-3′ | 520 | 57 |
| R:3′-TCCTTCCTTCTAGCTTTGG-3′ | |||
| CRYBB2 | |||
| Exon 6 | F:5′-CCCCTCGTTCACCCTCCCATCA-3′ | 506 | 69 |
| R:5′-CACTGTGTCCAAGGTCACACAGCTAAGC-3′ | |||
| CRYGC | |||
| Exons 1,2 | F:5′-TCAATCATATAGACAGAGCCA-3′ | 784 | 55 |
| R:5′-ATCTCCATCTAACCTTAGGT-3′ | |||
| Exon 3 | F:5′-AATGACAATTCCATGCCACA-3′ | 534 | 55 |
| R:5′-CCCACCCCATTCACTTCTTA-3′ | |||
| CRYGD | |||
| Exons 1,2 | F:5′-TGATAGCAATCCGAATACTCCA-3′ | 776 | 55 |
| R:5′-GGGTAATACTTTGCTTATGTGGGGAG-3′ | |||
| Exon 3 | F:5′-GTCCTCACCAAGCTGGACTG-3′ | 496 | 55 |
| R:5′-CCATTTGCCTCGTGTGTGTA-3′ | |||
| GJA8 | F:5′-AGGAGGTGAATGAGCACTCCA-3′ | 251 | 57 |
| R:5′-GTGCCCCACGTACATCAGG-3′ | |||
| MIP | F:5′-GAGGAGGTAACACTGTGGCAGC-3′ | 198 | 60 |
| R:3′-AGAAGCCAACGGCCAGG-3′ | |||
| BFSP2 | F:5′-GCTGCTGCACAAACAGTTGG-3′ | 286 | 62 |
| R:5′-TTCTGTTTCTAATGAGGTTGAACTTGTTA-3′ | |||
| GJA3 | F:5′-TGCAACACCCAGCAGCC-3′ | 474 | 60 |
| R:5′-GGCCACCGCCAGCAT-3′ |