Table 1 of Tröße, Mol Vis 2009; 15:1332-1350.

Table 1. Transcript names, short names, accession numbers, primer sequences, amplicon sizes, and fold change expression values obtained by microarray and qRT–PCR for selected transcripts.


Transcript name		 Short name Accession Number
Forward primer
Reverse primer		 Amplicon size FC by microarray FC by qRT–PCR
40S ribosomal protein S20 RPS20 BG936672 GCAGACCTTATCCGTGGAGCTA TGGTGATGCGCAGAGTCTTG 85

HSP90-beta HSP90B AF135117 CTCTGGGATGAGCTCCTCACA CCTTTGACCTCTTTGAGAACAAGAA 98

Elongation factor 1AA EF1AA AF321836 CCCCTCCAGGACGTTTACAAA CACACGGCCCACAGGTACA 57

Elongation factor 1AB EF1AB BG933853 TGCCCCTCCAGGATGTCTAC CACGGCCCACAGGTACTG 59

Metallothionein B MT-B CK990996 TGAATAAAGAAGCGCGATCAAA CTGGTGCATGCGCAGTTG 111 1.8 1.5*
Fatty acid binding protein FABP2 CB511332 TTACGGAAGGTGCTGGATTC GGGCATCAATGTGATGAAGA 108 −2.2 −2.5*
Gamma crystallin M2 CryG M2 CB510188 GGAGAAGATGTCACGGTGGT CCCCCTACAGAGGAGCCTAC 125 −1.8 −1.8*
Ependymin precursor EPN CA042089 TCCAGTTTAGCGTCCTGACA GACAAGACCGGCTGGATACT 104 −1.7 −2.3*
Fructose-bisphosphate aldolase B Aldo b CA043730 CCTGTGTGGTCATCTCTCCAT TGACAAGGGTGTCCTCTTCC 116 −1.7 −1.1
Sodium/potassium-transporting ATPase subunit alpha-1 precursor ATPA1C CA054630 AGGGAGACGTACTACTAGAAAGCAT CAGAACTTAAAATTCCGAGCAGCAA 85 1.4 1.5*
Calpain small subunit 1 CAPNS1 CB505442 AGGCACCCAATGAAGTTGTC CTCAGGGCTCATCTCCTCAC 144 1.4 1.5*
Glyceraldehyde-3-phosphate dehydrogenase GAPDH CA051897 GGTATCCCTTCATGGGTCCT CGTGTCAGTGGTGGACCTAA 104 1.2 1.8*
Ubiquitin-conjugating enzyme E2 D4 UbE2D4 CB508919 CATTGCATACTTTTGAGTCCAATC ACCCAGATGACCCCCTAGTC 101 −1.3 −1.4*
Cold-inducible RNA-binding protein Cirbp CA062835 TGAGCTTCGACACCACAGAG ACGAGACCTTCCCGTTTCTT 104 1.3 1.1
Peroxiredoxin-6 PRDX6 CA062947 TCAGGGATGTTTGGAGGAAC GGGGCGTAACTTTGATGAGA 126 −1.2 −1.1
SPARC precursor SPARC CA052160 CCAAGGCGATGTACTTGTCA GGTTCCTGTCCCACACAGAG 117 1.3 1.3*
Lens fiber membrane intrinsic protein LIM2 CA039129 CCAGGAAGTTCACCGTCACT TAATCGCAGGCATTCTCTCC 147 −1.3 −1.3*
Heat shock cognate 70 kDa protein HSC71 CA767816 ACCTCGTTGCACTTCTCCAG GCAGCGTGACAAGGTCTCTT 138 1.2 1.2*
Ferritin. heavy subunit Ferritin H CA052852 GTGTGGGTCGTTGTGTTCAG AGTGGGGTAGTGGTGTGGAG 110 1.2 −1
Phospholipid hydroperoxide glutathione peroxidase. mitochondrial precursor GPX4 CB505439 GGCTGTTCCTTCATCCACTT GCCAGGTACAGAGGTGGAAA 126 1.2 1.1

The first four rows in the table contain the selected reference genes that are not differentially expressed in lenses from the different dietary groups. Expression of the 16 transcripts in the subsequent rows was significantly different between the dietary groups when analyzed by microarray and SAM, but only 11 of the transcripts were significantly different when analyzed by qRT–PCR analysis and Mann–Whitney test (FCs are marked with an asterisk in the table).

Tröße, Mol Vis 2009; 15:1332-1350. http://www.molvis.org/molvis/v15/a141

©2009 Molecular Vision http://www.molvis.org/molvis/

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