Molecular Vision: Schmack, Mol Vis 2009; 15:146-161. Table 1

Table 1. Characteristics of the study and control groups

Mice			Injected material
Group	Genotype	Age (month)	Agent	Volume (µl)	Concentration
Study group 1	C57BL/6	2	RPE	50	0.5×10⁵/µl
Study group 2	C57BL/6	2	RPE+MB	50	0.5×10⁵/µl
Study group 3	Ccl-2^−/−	2	RPE+MB	50	0.5×10⁵/µl
Study group 4	C57BL/6	12	RPE+MB	50	0.5×10⁵/µl
Control group 1	C57BL/6	2	PBS	50	-
Control group 2	C57BL/6	2	MB	50	0.5×10⁵/µl

The table summarizes the characteristics of the four study and the two control groups and provides information on the subretinal injected agents. The animal groups were composed of 2-month-old C57BL/6 mice (study and control group 1+2), 12-month-old C57BL/6 mice (study group 4), and 2-month-old Ccl-2 knockout mice (Ccl-2^−/−, study group 3). Choroidal neovascularization membranes were induced by subretinal injection of 50 µl of retinal pigment epithelium (RPE) cells alone or RPE cells and microbeads (MB) with a concentration of 0.5x10⁵/µl each. The control mice received subretinal injections (50 µl) of phosphate buffer solution (PBS) or microbeads (0.5x10⁵/µl) Abbreviations: Ccl-2 knockout mice (Ccl-2^−/−); RPE represents retinal pigment epithelium (RPE); microbeads (MB); phosphate buffered solution (PBS); standard deviation (SD).