Figure 2. Illustration of the subretinal injection technique. A bent needle was inserted through a sclerotomy at 10 o’clock next to the limbus and pushed forward in a tangential direction toward the retina without touching the crystalline lens (A). Three to 21 days after inoculation, choroidal neovascularization membranes (asterisk) could be identified in the subretinal space next to the optic nerve by light microscopy (B, C). The retinal (C) and sceral (D) insertion sites are highlighted by arrows. B-D: Periodic acid-Schiff (PAS) staining was used and original magnifications are 4X (B) and 20X (C,D).