Figure 7 of Barathi, Mol Vis 2009; 15:1277-1293.

Figure 7. Effect of muscarinic antagonists in SF cell proliferation. Cell proliferation of mouse sclera fibroblasts were pre-incubated with atropine, pirenzepine at concentrations of 0.1, 1, 5, 10, and 100 μM, and MT-7 at 0.1, 1, 2, 4 μM for 3 h before they were incubated with equimolar carbachol at 0.1–100 μM and MT-1 at 0.1, 1, 2, 4 μM. BrdU incorporation was measured 24 h later by ELISA. Cells were treated together with antagonists and agonists. The antagonists blocked the agonist’s activation in a dose-dependent manner (p<0.05, ANOVA, n=4). Data represent mean±SEM. The asterisk indicates p<0.05 versus control (Post Hoc Analysis; Tukey Honest Significant Difference).