Figure 5. Muscarinic receptor protein expression. A: Immunoreactive bands corresponding to each muscarinic receptor subtype in mouse scleral fibroblasts and their estimated molecular weights are shown: ~58 kDa (M₁), ~52 kDa (M₂), ~52 kDa (M₃), ~66 kDa (M₄), and ~65 kDa (M₅). Blots are representative data from at least three independent experiments. B: Immunoreactive bands corresponding to each muscarinic receptor subtypes in human scleral fibroblasts and their estimated molecular weights are shown: ~57 kDa (M₁), ~52 kDa (M₂), ~56 kDa (M₃), ~58 kDa (M₄), and ~51 kDa (M₅). Blots are representative data from at least three independent experiments. Molecular standards (50-75 kDa) were run on the same blot parallel with muscarinic receptor proteins. The positions are shown in the left hand side.