Figure 10. Activation of ERK1/2 and Ras by 50 mg/ml carbachol in cultured mouse scleral fibroblast cells. Cultured mouse SF cells were serum starved for 24 h in DMEM and then incubated with 50 mg/ml of carbachol for 0.5, 1, 2, and 6 h as well as overnight. Time-dependent change of phospho-p42/44 MAPK and Ras in the presence of 50 mg/ml of carbachol was detected by immunoblot analysis.