Figure 1 of Barathi, Mol Vis 2009; 15:1277-1293.

Figure 1. Immunohistochemistry of muscarinic receptor subtypes in early passages of cultured human and mouse scleral fibroblasts. A is the image of the early passage of cultured human fibroblasts, and B is the image of the early passage of mouse scleral fibroblasts. Subtype selective antibodies bound to cultured cells demonstrated the presence of the muscarinic receptors M1-M5 (shown in green). When primary antibodies were omitted, no binding was observed (negative control). The M1-M5 receptors were localized to the cell membrane as well as to the cytoplasm. Magnification, 200X. All experiments were performed in triplicate.