Figure 1. Immunohistochemistry of muscarinic receptor subtypes in early passages of cultured human and mouse scleral fibroblasts. A is the image of the early passage of cultured human fibroblasts, and B is the image of the early passage of mouse scleral fibroblasts. Subtype selective antibodies bound to cultured cells demonstrated the presence of the muscarinic receptors M₁-M₅ (shown in green). When primary antibodies were omitted, no binding was observed (negative control). The M₁-M₅ receptors were localized to the cell membrane as well as to the cytoplasm. Magnification, 200X. All experiments were performed in triplicate.