Figure 4. Influence of LOR on the histopathological and immunohistochemical studies in mouse corneas 14 days after UV irradiation. The typical histologic findings of cornea stained with hematoxylin and eosin (A, B) are shown. A: The cornea in the saline-treated group shows marked inflammation, obvious edema, profound neovascularization, and significant hypercellularity in the stroma. B: The cornea in the LOR-treated group exhibits only scattered inflammatory cells, mild stromal swelling, and less neovascularization. Corneal tissues (C-F) were analyzed by immunohistochemistry to determine the expression of NF-κB. Immunohistochemical staining with an antibody against activated NF-κB was performed to detect the expression of NF-κB. Sections incubated without a primary antibody served as negative controls. All tissue sections were counterstained with hematoxylin. These samples were representative of all corneas examined. Brown staining indicates activated NF-κB. C,D: The cornea in the mock-infected group and the cornea in LOR alone group show that NF-κB activity is only observed very faintly in the base cells of epithelium (arrowheads). E: Recurrence induced wide spread positive staining of NF-κB, which was most robust in the stroma (arrows) of the saline-treated group. F: Scant immunoreactivity of NF-κB was observed in the stroma of the LOR-treated group (arrows). Original magnifications, 400X.