Figure 3. CP55,940 attenuates H₂O₂-induced cytotoxicity and ROS generation. A: Protective effects of CP55,940 on H₂O₂-induced decrease in cell viability measured by the MTT assay. Cells were pretreated with 0–1 μM CP55,940 for 15 min before exposure to 200 μM H₂O₂ for 24 h, and cell viability was measured by MTT assay. Asterisk (*) the correlation significant at the p<0.05 level and suggest a significant increase in cell viability as compared to H₂O₂-treated group. B: Protective effects of CP55,940 on H₂O₂-induced increase in intracellular ROS in ARPE-19 cells. Cells were pretreated with 0–1 μM CP55,940 for 15 min before exposure to 200 μM H₂O₂ for 24 h, and intracellular ROS was measured by the DCF-DA assay. Aterisk (*) represents the correlation significant at the p<0.05 level and suggest a significant decrease in intracellular ROS generation as compared to H₂O₂-treated group.