Figure 4. Effect of P2Y₂-R siRNA on Ap₄A-induced migration of corneal epithelial cells. SIRC cells were incubated for 72 h with transfection reagent alone (control), P2Y₂-R siRNA #1, or P2Y₂-R siRNA #2 and then wounded with a pipette tip in the presence or absence of Ap₄A. The graphs (A and B) show the variation of the wounded area versus time and the estimated migration rates (EMR) of cells transfected with siRNA 1 and siRNA 2, respectively, in the presence of Ap₄A (P2Y₂-R siRNA+Ap₄A). Ap₄A accelerated the rate of healing in cells transfected with transfection reagent alone (control+Ap₄A) compared with the rate of both P2Y₂-R siRNA-transfected cells in the presence of Ap₄A (P2Y₂-R siRNA+Ap₄A). Three asterisks indicate p<0.0001 when compared to the control, and two asterisks indicate p<0.001 when compared to Ap₄A alone.