Figure 4. Effect of P2Y2-R
siRNA on Ap4A-induced migration of corneal epithelial cells.
SIRC cells were incubated for 72 h with transfection reagent alone
(control), P2Y2-R siRNA #1, or P2Y2-R
siRNA #2 and then wounded with a pipette tip in the presence or absence
of Ap4A. The graphs (A and B) show the
variation of the wounded area versus time and the estimated migration
rates (EMR) of cells transfected with siRNA 1 and siRNA 2,
respectively, in the presence of Ap4A (P2Y2-R
siRNA+Ap4A). Ap4A accelerated the rate of healing
in cells transfected with transfection reagent alone (control+Ap4A)
compared with the rate of both P2Y2-R
siRNA-transfected cells in the presence of Ap4A (P2Y2-R
siRNA+Ap4A). Three asterisks indicate p<0.0001 when
compared to the control, and two asterisks indicate p<0.001 when
compared to Ap4A alone.