Figure 5. Nucleostemin expression and cell size in primary culture. Cell size was different in early passage (P1-P3) of primary mouse
corneal culture, therefore those cells were used for analyzing correlation between cell size and nucleostemin expression.
A: Immunostaining in P2 showed that small cells were strongly positive in nucleostemin, but negative in large cells. All of
the cells with the cell size of smaller than 100 μm expressed nucleostemin (B and C), however cells with the size of larger than 100 μm showed less or no expression of nucleostemin (B and D).